SACCHAROMYCES CEREVISIAE YEAST CELLS AS A MODEL FOR STUDYING PORTEÍNAS IMPLICATED IN HUMAN DISEASES.Author:
RODRÍGUEZ ESCUDERO M. ISABEL.
Year:
2004.
University:
COMPLUTENSE DE MADRID [
www.ucm.es].
Place of defense: FACULTAD DE FARMACIA.
Place of preparation: FACULTAD DE FARMACIA , UNIVERSIDAD COMPLUTENSE.
Summary: The study of the virulence of a number of pathogenic bacteria has revealed a strategy common infection based on the use of the signal transduction pathways of the cell itself hospedadora.La vast majority of molèculas signaling human cells bacterial proteins that interact with, such as the family GTPasas RHO or MAPK, show homology to proteins of the yeast S.cerevisiae.Esto opens the possibility of using this unicellular eukaryotic organism as a model for the study of bacterial proteins, and thus have a very manageable system for identifying new virulence factors, defining their interaction with the host and, finally, to test possible inhibitor drugs capable of blocking infection in humans and animales.En this thesis we have confirmed the usefulness of this model studying the role of protein secret by the type III secretion system of Salmonella Typhimurium (SIGD / SOPB) and the enteropatógena Escherichia coli (EPEC) (ESPA ESPB, ESPD, ESPD, ESPF, ESPG, ESPH, MAP AND IRR) . addition, we used this model for the study of proteins associated with this process tumor model for the study of proteins associated with human tumor processes, such as those belonging to the route of fosfatidilinositol 3-quinasa (PI3K) -proteína B ( PKB / AKT). In this line, we studied using this model PI3K, its main effector Akt and its antagonist functional PTEN, as well as its counterpart in yeast, Tep1.
