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14 theses in 1 pages: 1
  • SYSTEMATIC ANALYSIS AND MOLECULAR MITES IXODOIDEOS INTEREST HEALTH.

    Author: VITUTIA GARCÍA M. MAR.
    Year: 2003.
    University: COMPLUTENSE DE MADRID [More theses of this university] [www.ucm.es].
    Place of defense: FACULTAD DE VETERINARIA.
    Place of preparation: FACULTAD DE VETERINARIA.
    URL: http://www.kriptia.com/en/CIENCIAS_DE_LA_VIDA/ZOOLOGIA//PARASITOLOGIA_ANIMAL/1#107745
    Summary: The suborder Ixodida consists of a heterogeneous group of parasites hematógrafos (ticks). In this paper we have used eight species representing the majority of taxon two families, which were selected because they are proven or potential vectors of disease agents that affect the man or animales.Uno fundamental objective of the work has been to study in depth the evolutionary relationships among the various taxa of the suborder, starting six nucleotídicas mitochondrial sequences. This secuenció the control area and adjacent regions of the mitochondrial DNA of the species listed and analyzed structurally genes identificados.Con molecular data obtained were performed reconstruciones phylogenetic by different methods and assumptions were evaluated obtained. The results detected that the order of genes section Prostriata is maintained by gender Árgas and confirm the genomic organization mitocondríal particular group Metastriata.Las assumptions provided phylogenetic general groupings conincidentes with traditional taxonomic organization divided into two families Argasidae and Ixodidae. The streams in the region to control the species analyzed provide a high power for further analytical studies and epidemiological populations.
  • ANGUILLA ANGUILLA HELMINTHS PARASITES L. IN THE ESTUARIES OF AROUSA AND FERROL.

    Author: SAN MARTÍN OUTEIRAL SARA.
    Year: 2004.
    University: SANTIAGO DE COMPOSTELA [More theses of this university] [www.usc.es].
    Place of defense: FACULTAD DE FARMACIA.
    Place of preparation: FACULTAD DE FARMACIA.
    URL: http://www.kriptia.com/en/CIENCIAS_DE_LA_VIDA/ZOOLOGIA//PARASITOLOGIA_ANIMAL/1#107906
    Summary: It has conducted a study analyzing 956 eels parasites, caught in the estuaries of Arousa (n = 477) and Ferrol (n = 479) between January 1995 and December 1996. The species have been found: a) the monogéneo Pseudodactylogyrus anguillae b) digéneos Bucephalus anguillae, Lecithochirium musculus, L. Rufoviride, L. Furcolabiatum, Lecithaster gibbosus, Derogenes varicus, Deropristis inflata, Helicometra fasciata and Podocotyle angulata c) tapeworms Grillotia sp. Plerocercoide, Tetraphyllidea fam, gene sp "Bothriocephalus claviceps and Proteocephalus macrocephalus d) nematodes Cucullanus heterochrous, Contracaecum sp. Larvae, Cystidicolidae gene, sp, larvae, Spinitectus inermis and Capillariinae gene. Sp; e) acanthocephalans Acanthocephaloides propinquus and Acanthocephalus clavula. The most important species in terms of their prevalence, mean abundance and average intensity were inflata D., H. fasciata, L. musculus p, angulata. studies were carried out according to the estuary comparative statistical sampling, the size and weight host, seasonal and area specific location in the digestive tract. have also been established correlations between the presence and intensity of the different species of helminths. Species A. propinquus and L. furcolabiatum are more likely to be in eels from the Arousa estuary that in the coming Ferrol. Moreover, there are statistically significant differences in the intensity between the two rias for species inflata D., P. angulata, B. anguillae, L. musculus and H . fasciata. Having analyzed the influence of height and weight in the parasitación host, it has been observed that the risk of a host species is parasitado by musculus L. and H. fasciata is proportional to the increase in its size. De the mísma way, there is a positive correlation between the size of the host and intensity of parasitación for species L. furcolabiatum and P. angulata; intensity of the latter kind also shows positive correlation with the weight of the host. was deteminado existence significant differences between the seasons for some digéneos, although the stations at presenting its maximum intensity varies with the species. species found in the stomach show preference for blind stomach area. Species intestinal D. inflata, H. fasciata and P. angulata manifest tendency to occur in specific areas of the intestine, also presenting significant differences between all areas. Lastly, there is a positive correlation between the presence and intensity certain digéneos as musculus L. and H. fasciata, and moreover, between acantocéfalo A. propinquus and digéneos L. rufoviride, L. musculus, H. fasciata and B. anguillae.
  • PARASITISM OF MARINE FISH, ALL BIOLOGICAL, ECOLOGICAL AND SYSTEMATIC STUDY OF THE SPECIES (BUCEPHALOIDEA)

    Author: MEDINA VOLTES MANUEL.
    Year: 2004.
    University: LA LAGUNA [More theses of this university] [www.ull.es].
    Place of defense: FACULTAD DE FARMACIA.
    Place of preparation: FACULTAD DE FARMACIA.
    URL: http://www.kriptia.com/en/CIENCIAS_DE_LA_VIDA/ZOOLOGIA//PARASITOLOGIA_ANIMAL/1#110255
    Summary: Parasitism was studying marine fish, the Superfamily Bucephaloidea with redescripcion thirteen species Helminths, located in a total of 2204 fish have been reviewed sampled waters of the Canary archipelago, bringing seven new species for helmintofauna Spanish, and three new species of fish as new definitive hosts. Also confirming the validity of Folliculovarium gimnotoraci, Bucehalopsis callicotile and Labratrema minimus. There will always be a comparative study between the parasitofauna of Hawaii Island and the Canary Islands. The subfamily Bucephalinae is represented by six species of marine fish in the Canary Islands The subfamily Prosorhynchinae by four species and the subfamily Dolichoenterinae two species and Folliculovarinae by a species. It accompanies a study in the area of providing sampling aspects of the biological and larval stages of the known biological cycles, as well as ecological and systematic aspects of the species studied.
  • CELLULAR AND HUMORAL IMMUNE RESPONSES TO IBERIAN PIG METASTRONGILOSYS

    Author: ALCAIDE ALONSO MARÍA.
    Year: 2004.
    University: EXTREMADURA [More theses of this university] [www.unex.es].
    Place of defense: FACULTAD DE VETERINARIA.
    Place of preparation: FACULTAD DE VETERINARIA.
    URL: http://www.kriptia.com/en/CIENCIAS_DE_LA_VIDA/ZOOLOGIA//PARASITOLOGIA_ANIMAL/1#110595
    Summary: The metastrongylosis is one of the parasites that most affects pigs reared in extensive. This nematodosis lung, for many years has been forgotten by the connotations of its life cycle, and that is whether the presence of earthworms for transmission, were inconsistent with the tecnificadas facilities required for the operation of the pig races early. But the resurgence, with more force than ever, the traditional breeding Iberian pig has caused this parasitism is at the forefront of the health picture of the pig farms. With the development of this experience, we intend to dig deeper into the impact of multiple organ at the metastrongylosis in pigs, especially in the valuation of the cellular and humoral immune response that developed pigs against the parasite, in order to assess the degree of resistance to the different treatments followed in the experiment. To that end, we used 48 pigs race Iberian divided into five groups, which were applied various patterns of infection Metastrongylus apri. We tried to reproduce experimentally, different situations infection that may occur in nature, from simple infections, 3000 L3 (Lot 1) and 1000 L3 (Lot 2), until infections with low doses successive (200 +400 +800 L3) and subsequent challenge with 1000 L3 (Lot 3). Lot 4 included animals that were immunized with different antigenic excerpts of the parasite and also challenged with 1000 L3. Finally, lot 5 was formed by a group of animals used as a control uninfected. Three animals from each group were sacrificed on day O, 14, 35 and 84 post-infection .. We conducted a comparative study between different experimental groups of symptoms, from macroscopic lesions in the lungs and liver of the histopathology in lungs, liver and lymph nodes, the number of larvae and adults present, their morphology and localization in the lung, the number of eggs removed with faeces and differential counts of eosinophils in the blood. To complete this analysis, were used techniques such as ELlSA and flow cytometry, in order to assess the humoral and cellular immune response, both systemic and local levels. The first clinical signs, cough, abdominal breathing or breathlessness, were recorded between days 10 and 12 post-infection. The intensity and frequency with which they occurred, primarily cough, was positively correlated with the infective dose applied. Likewise, it was shown that the progressive reinfections with small dosage and immunization inócu! You produced from adults and larvae of the parasite, greatly reduced the severity of symptoms caused by this parasite. The pulmonary lesions described as emphysema, atelectasis, pneumonia or granulomas, were significantly milder in animals reinfected. Overall, the lung injury caused by M. Apri, can be fit inside a typical pneumonia with parasitic tendency to chronicity. In lymph torácico-pulmonares in all experimental groups, there was a process characterized by hyperplasia and hypertrophy of lymphoid follicles, which had a highly developed center germination and low crown follicular, own follicles very active. The hepatic lesions were most relevant milk stains, which are a reaction mechanism of the body against the erratic migration of larval state. The parasite possessed a high capacity development in the lung tissue of pigs, especially in groups primoinfectados, where the rate rec 8 uperació ad6 No ranged between 18-20%. In the batch of animals reinfected, is not exceeded el1%, which showed the important resistance gained by them after the previous contact with successive doses of M. Apri. Regarding the immunized group were observed results very similar to those found in lots 1 and 2, although a day 84 after infection, there was a reduction of 50% of the population of worms present in the lung. It was found that the intensity of the signs clinical, pathologic lesions and lung strength parasitación were dose-related. The proportion female / male was significantly higher in animals reinfected respect to primoinfectados, showing that sex dominant parasitic processes with limited number of individuals were females. This phenomenon noted the important survivability of the parasitic species in adverse conditions. The length of the adult females of M. Apri, was closely related to its ability to accommodate eggs, because there was a positive correlation between the average length of the females and the concentration of eggs in the feces. Mean concentration of eggs in the feces of infected animals with a high dose of L3, were the highest recorded in all the other groups, followed by the animals lots 2, 4 And finally, from 3. In addition, significant positive correlations were found between the number of adults present and the load of eggs in feces. Injuries more features observed in the pulmonary parenchyma were the presence of areas atelectásicas areas enfisematosas compensatory and a major infiltration cell fagociticas from 14 dpi. In the intermediate and final stages of the studies showed the presence of granulomas parasitic, so the table defined lesion in the lungs of infected animals come within a process neumónico typical parasite with a tendency to chronicity. All experimental groups showed the highest number of liver injuries a day 35 after infection. The presentation of milk spots in the liver responds to the development of resistance to the parasite. This relationship between responsiveness and pathogenesis liver, it is.
  • REGULATING THE PRODUCTION OF NITRIC OXIDE MACROFAGICA BY DIFFERENT SPECIES OF HELMINTHS.

    Author: ANDRADE MARIA DO AMPARO.
    Year: 2004.
    University: SALAMANCA [More theses of this university] [www.usal.es].
    Place of defense: FACULTAD DE FARMACIA.
    Place of preparation: DFACULTAD DE FARMACIA.
    URL: http://www.kriptia.com/en/CIENCIAS_DE_LA_VIDA/ZOOLOGIA//PARASITOLOGIA_ANIMAL/1#111428
    Summary: The infection caused by parasitic organisms causing changes in the physiology of the host, which can either promote or undermine it. One of these changes is reflected in the production of nitric oxide (NO) by host macrophages in response to certain stimuli parasite. The overall objective of this project was to assess the effect doctoral on production by alveolar macrophages ON practicing various antigens (crude or purified molecules), obtained six genres helminths (Echinococcus, Paragonimus, Schistosoma, Trichinella, Dirofilaria and Ascaris) involved in the lesion from different structures of the respiratory system conclusions obtained: Both antigens from cystic wall (PQ) E. Multilocularis and E. Granulosus as antigens derived from the laminar layer (CL) of E. Multilocularis inhibit the production of ON stimulated by rat alveolar macrophages. The recombinant protein 14-3-3 E. Multilocularis is one of the components responsible for the suppressive effect observed by antigens from the CL and PQ heartworms. . Antigens excretory / secretory (E / S) from the flukes P. Mexicanus S. Bovis behave differently modulate the production of ON, being only derivatives P. Mexicanus which stimulates the production of ON. - There are differences between species and non-cystic cystic Trichinella in its ability to stimulate the production of ON. - A molecule 35.3 kDa, isolated from adult somatic antigen D. Immitis and belonging to the immunoglobulin superfamily, stimulates the release of ON. The antigen-E / S obtained both larval stages and adult stages of A. Suum are able to stimulate the production of ON. Specifically, a fraction of PM between 37-46 kDa, antigen E / S Adult A. Suum, is involved in its production.
  • EVALUATION OF THE PROTECTIVE CAPACITY OF RECOMBINANT MOLECULES OF FASCIOLA HEPATICA.

    Author: CASANUEVA GOMEZ PATRICIA.
    Year: 2004.
    University: SALAMANCA [More theses of this university] [www.usal.es].
    Place of defense: FACULTAD DE BIOLOGIA.
    Place of preparation: FACULTAD DE FARMACIA.
    URL: http://www.kriptia.com/en/CIENCIAS_DE_LA_VIDA/ZOOLOGIA//PARASITOLOGIA_ANIMAL/1#111438
    Summary: This work raises have an effective vaccine against fasciolosis, animal and human disease caused by trematodo Fasciola hepatica. We tested the use of protein combined with fatty acids (FABP) and recombinant peptides derived from these in new systems adaptación-adyuvados, and measure if they increase protection levels obtained with previous systems. The steps were followed in the development of this work were: obtaining and purification of recombinant molecules coupled with fatty acids from Fasciola hepatica; design and expression of peptides recognized by T lymphocytes derived from the molecule recombinant Fh15; were tested with these new molecules products adyuvados incorporating immunomodulatory not toxic; was then conducted a security assessment with different protocols immunization and infection, using different experimental models (rabbit, lamb and mouse) and finally studied the immune response triggered by the implementation of vaccine analyzing your balance Th1-Th2 and its evolution over the experiments. Once the experiments, we observed that attained a high level of protection in rabbits immunized with Fh15, that effect is enhanced by increasing the time interval between vaccination and infection; The positive effects from vaccination with Fh15 in sheep model consist of deficiencies in the physical and biological control of the worms, while decreasing the size of the parasite as a delay in maturation and decrease in the number of eggs produced. Experiments using a recombinant peptide derived from Fh15, merged with glutathione -S- transferase show adequate levels of protection, similar in three different experimental models. High levels of IgG2a detected in vaccinated mice survived both Fh15 with the peptide recombinate T51-62 suggest that protection mechanisms induced by this vaccine are due to the predominance of responses Th1.
  • HELMINTOFAUNA OF LACÉRTIDOS ENDEMIC IN THE CANARY ISLANDS: RELATIONSHIPS PARÁSITO - HOSPEDADOR

    Author: MARTIN JUAN EMILIO.
    Year: 2004.
    University: VALENCIA [More theses of this university] [www.uv.es].
    Place of defense: FACULTAD DE FARMACIA - UNIVERSITAT DE VALÈNCIA.
    Place of preparation: FACULTAD DE CIENCIAS BIOLÓGICAS.
    URL: http://www.kriptia.com/en/CIENCIAS_DE_LA_VIDA/ZOOLOGIA//PARASITOLOGIA_ANIMAL/1#111885
    Summary: The present study has been carried out analysis helmintofaunístico of 8 species and subspecies host belonging to the genus Gallotia (Sauria: Lacertidae) in the Canary Islands, specifically: G. Stehlini (Gran Canaria), G. Caesaris caesaris (Iron), G. C. Gomerae (La Gomera), G. Galloti galloti (Tenerife), G. G. Palmae (La Palma), G. Atlantica atlantica (Lanzarote), G. A. Laurae (Lanzarote) and G. A. Mahoratae (Fuerteventura). In total, were found 23 species of helminths: 1 Digenea (Pseudoparadistomum yaizaensis), 5 Cestoda (Mesocestoides sp. Dipylidium sp. Diplopylidium acanthotetra, Oochoristica agamae and Nematotaenia tarentolae), 16 Nematoda (Thelandros filiformis, Thelandros galloti, Thelandros tinerfensis , Alaeuris numidica canariensis, Tachygonetria dentata, Tachygonetria macrolaimus, Tachygonetria cone, Tachygonetria numidica, Parapharyngodon micipsae, Parapharyngodon echinatus, Parapharyngodon bulbosus, Spauligodon atlanticus, Skrjabinelazia pyrenaica, Skrjabinelazia hoffmanni, Skrjabinodon medinae and Spirurida gene. sp.), and 1 Acanthocephala (Centrorhynchus sp.). The analysis reveals differences in the structure and composition of the communities helmintianas these lizards canaries, both among themselves and in relation to other lacértidos island and mainland. These differences are primarily related to: (i) the existence of endemic species of helminths, (ii) the presence of nematodes Pharyngodonidae typical reptile herbivores, in all hosts except in the easternmost islands (G. atlantica), and further, in the larger lizards (G. stehlini), (iii) the existence of communities helmintianas with a tendency toward high diversity in terms of wealth and abundance of species, mainly in the hosts larger and in the higher content of plant material in their digestive tracts (G. stehlini and G. galloti palmae).
  • STUDIES ON THE LIFE CYCLE AND TRANSMISSION ENTEROMYXUM SCOPHTHALMI (MYXOZOA), PARASITE ENTÉRICO TURBOT

    Author: REDONDO GAMERO MARIA JOSE.
    Year: 2005.
    University: VALENCIA [More theses of this university] [www.uv.es].
    Place of defense: FACULTAD DE FARMACIA.
    Place of preparation: FACULTAD DE FARMACIA (UNIVERSITAT DE VALENCIA ESTUDI GENERAL).
    URL: http://www.kriptia.com/en/CIENCIAS_DE_LA_VIDA/ZOOLOGIA//PARASITOLOGIA_ANIMAL/1#111683
    Summary: Enteromyxum scophthalmi parasitizes the digestive tract turbot producing a serious illness emaciativa known as "syndrome plunged head," causing high mortality, with the consequent impact on the cultivation of this kind. For effective prevention of enteromixosis, it is necessary to the understanding of the epidemiology, life cycle and transmission of the parasite, aspects that are discussed in this paper. Although it was not possible to achieve the continuous in vitro cultivation of E. Scophthtalmi, found that the stadiums can remain viable until 12 days amid M199 and 24 hours in seawater. It was demonstrated by experimental transmission cohabitation fish healthy and infected by contaminated by effluent stages of the parasite and orally, and epidemiological data were obtained in monitoring natural infection on the farms. The study of fish naturally or experimentally infected, as well as the use of an in vitro model in the presence of intestinal tissue explants, allowing detailed information about the transmission and development of mixosporidio. The stadiums in the various stages of infection were classified in early stages and proliferative (ST1, ST2 and ST3) and stadiums esporogónicos (ST4 and ST5), which were characterized morphologically (optical microscopy and electronics), and by histochemical techniques, including hybridization spot. These results established a tentative model of the life cycle and transmission of the parasite, and identified the main risk factors for enteromixosis growing turbot. Data collected on the entry and dispersal of infective stages are useful to devise prevention and control measures to prevent the introduction and spread of mixosporidio on farms growing, and their release into the environment.
  • SYSTEMATIC, FAUNISTIC AND ECOLOGYC STUDY OF THE PARASITIC HELMINTH FAUNAS OF TALPIDS IN THE SOUTH WESTERN EUROPE.

    Author: RIBAS SALVADOR ALEXIS.
    Year: 2005.
    University: BARCELONA [More theses of this university] [www.ub.es].
    Place of defense: FACULTAD DE FARMACIA.
    URL: http://www.kriptia.com/en/CIENCIAS_DE_LA_VIDA/ZOOLOGIA//PARASITOLOGIA_ANIMAL/1#112845
  • STUDIES AMOEBAS LIFE FREE OF GENDER ACANTHAMOEBA IN THE CANARY ISLANDS

    Author: LORENZO MORALES JACOB.
    Year: 2005.
    University: LA LAGUNA [More theses of this university] [www.ull.es].
    Place of defense: DEPARTAMENTO DE PARASITOLOGIA, ECOLOGIA Y GENÉTICA.
    Place of preparation: UNIVERSIDAD DE LA LAGUNA.
    URL: http://www.kriptia.com/en/CIENCIAS_DE_LA_VIDA/ZOOLOGIA//PARASITOLOGIA_ANIMAL/1#116849
    Summary: This paper presents a study of epidemiological and molecular gender acathamoeba in subtropical environments (Canary) and tropical (Jamaica). Water samples were analyzed for domestic use, sea water, river water and soil, in areas related to human activities. The ambebaas isolated were cultivated for its characterization, identifying their species / genotype. For the correct identification of isolates obtained, it conducted a series of tests based on molecular chain reaction (PCR) the polymerases. First, it isolates obtained were subjected to PCR using primers for generic gender acanthamoeba. Subsequently isolates that gave positive amplification, underwent PCr using primers specific to the species A. Astronyxis, A. Divionensis and A. Polyphaga. All isolates classified as belonging to the genus Ãcanthamoeba were characterized from the point of view of their ability pathogenic and virulent, which primers were used to amplify a fragment which only presents the pathogenic amoebas, and primers for serin-proteadas extracellular. Finally, all isolates were genotipados, using the region diagnosed DF3 of the small subunit of RNA Ribosom the (ssurRNA), which allows the group to isolated groups in the so-called T, from T1 to T15. The results concluded that the amoebas related areas of stress and thermal osmótico showed a greater degree of pathogenicity and virulence. Following the development of molecular techniques for the identification of species of Acanthamoeba in the universtiy of the west indies, Kingston, described the first case was diagnosed Acanthamoeba keratitis in Jamaica, being the kind A. Polyphaga, responsible for the infection, which shows that Acanthamoeba is a patógeno emerging that can cause diseases in the tropics. Among the factors Virulence of Acanthamoeba, it is the role of study as serin-proteasas extracellular these amoebas, this will use RNA interference as a tool for silencing of the catalytic domain of these proteases. To verify the effectiveness of silencing were studied the activities of these proteases by zymogrammes protease, measurement activities delas proteases delos supernatants of crops, and conducted tests cytotoxicity and analysis of the levels of messenger RNA (mRNA) from Serin-Proteasas extracellular and both of amoebas and not treated as those treated with RNAi from clinical samples and environmental varying virulence. After treatment, there was a reduction in the levels of extracellular proteases, cytotoxicity and genetic levels of mRNA in pathogenic strains and moderately pathogenic. Demonstrating that serin-proteasas extracellular are directly involved in the pathogenesis and virulence of acanthomoeba.
  • DEVELOPING A VACCINE AGAINST LEISHMANIASIS.

    Author: PEREZ JIMENEZ EVA.
    Year: 2005.
    University: AUTÓNOMA DE MADRID [More theses of this university] [www.uam.es].
    Place of defense: FACULTAD DDE CIENCIAS.
    Place of preparation: FACULTAD DE CIENCIAS.
    URL: http://www.kriptia.com/en/CIENCIAS_DE_LA_VIDA/ZOOLOGIA//PARASITOLOGIA_ANIMAL/1#121664
    Summary: Leishmaniasis The term encompasses a group of parasitic diseases caused by different species of protozoa of the genus Leishmania. The disease is endemic in tropical and subtropical regions in 88 different countries in Asia, Africa, Europe and America, 72 of which are developing countries. According to the World Health Organization, 350 million people are at risk of contracting the disease. It is estimated that the global prevalence is 12 million people affected. Despite treatment with drugs, the emergence of resistant variants of the same parasite is becoming increasingly common. The fact that after a infecicón natural Leishmania spp. Was acquired immunity against subsequent infection, suggests that the prophylactic immunization is feasible. However, there is still no effective vaccine. The development of a vaccine able to confer long-term immunity against the parasite is crucial for the control of leishmaniasis. An optimal vaccine must induce both T cells CD4 + T CD8 + specific to the parasite. In this paper, we have reviewed various strategies to modulate the immune response induced by using protein LACK of L, infantum as antigen, as well as the effectiveness to confer protection against cutaneous and visceral forms of the disease in the mouse model. The immunization protocols based on an initial dose with a vector DNA which expresses the protein LACK (ADN-LACK) followed by a second dose with a vaccinia virus (VV), which expresses the same protein, have shown to confer partial protection to the front infection L.major. To improve the immunization protocol, first studying whether Interleukin 12 (IL-12) and IL-18 could improve the protection obtained. We found that immunization with an initial dose with ADN-LACK along with two arrays of DNA encoding cytokines IL-12 and IL-18, followed by a second dose with a recombinant vaccinia virus expressing LACK, induced an immune response of type Th1 and conferred a high level of protection against infection with L.major. Because of the safety concerns arising from the use of live vaccines based viruses replicativos, the next goal that we set was to generate a recombinant vaccinia virus based on the highly attenuated MVA strain expressing the protein LACK of L.infantum (MVA-LACK) . In this paper we have demonstrated that immunization based on an initial dose with ADN-LACK followed by a dose MVA-LACK induces a greater number of T cells CD8 + secreting IFN-gamma well as higher levels of IFN-gamma in the supernatants of the splenocytes restimulados with protein LCAK compared with immunization with the virus replicativo VV-LACK. Furthermore, protection from the face of infecicón by L.major was significantly higher in animals immunized with AD-LACK/MVA-LACK to be immunized with the same viral doses. This protection is maintained over time for at least 17 weeks and in turn correlates with higher levels of T cells CD8 + specific secreting IFN-gamma and TNF-alpha. To determine if our protocol immunization could induce protection against various forms of the disease evaluate the effectiveness of this protocol to the immunization against visceral leishmaniasis caused by L.infantum. One month after infection with the parasite, animals vaccinated with the protocol immunization heterologous ADN-LACK/Virus vaccinia recombinant showed high levels of protection, which were comparable in both animals immunized with MVA-LACK with VV-LACK , which supports the use of viruses attenuated MVA-LACK as vaccine against the disease. Our data show a clear advantage virus MVA-LACK spent on immunizations on heterologous virus replicativo VV-LACK as a vector vaccine. Therefore, MVA-LACK is a promising candidate vaccine for use in controlling the lesihamniosis.
  • CHARACTERIZATION GENOTYPIC SPECIES SUBCOMPLEJO INFESTANS (HEMIPTERA-REDUVIIDAE) BASED ON SEQUENCES MARKERS ADN RIBOSOMAL NUCLEAR AND MITOCHONDRIAL.

    Author: KLISIOWICZ DEBORA DO ROCIO.
    Year: 2006.
    University: VALENCIA [More theses of this university] [www.uv.es].
    Place of defense: FACULTAD DE FARMACIA.
    Place of preparation: FACULTAD DE FARMACIA.
    URL: http://www.kriptia.com/en/CIENCIAS_DE_LA_VIDA/ZOOLOGIA//PARASITOLOGIA_ANIMAL/1#115600
    Summary: Chagas disease or Tripanosomosis Americana is a disease of high importance in most countries Americans being the elimination of vectors the only effective way to attempt to interrupt the cycle of this disease. This paper aims, by sequencing the region intergénica of nuclear ribosomal DNA (ITS-1, 5.8Se ITS-2) and the gene mitochondrial NADH desidrogenase 1 (ND1) study subcomplejo infestans with the aim of understanding the process expansion and location of these species and provide tools that can help in the systematic, epidemiology and control of this group of insects. For this study were analyzed 95 copies of that subcomplejo, including Triatoma infestans (77), Triatoma melanosoma (2), Triatoma platensis (4) and Triatoma delpontei (12). The markers ITS-1 and ITS-2 allow the distinction between species and populations of subcomplejo infestans. This is the first time that describes a mitochondrial gene (ND1) full species subcomplejo infestans introducing this gene nucleotide variability greater than the region intergénica. The two markers used in this study were excellent as molecular markers for subcomplejo infestans and separated populations  ¨ andinas ¨ T. Infestans of  ¨ not andinas ¨. These markers also confirm that insects referred to as T. Melanosoma and option chromatic  ¨ dark-morph ¨ T. Infestans are to be held within the species T. Infestans. The phylogenetic analysis with ribosomal markers clearly separates the different species of subcomplejo infestans while, with the mitochondrial show a topology not coincident with the region intergénica noting that the evolutionary history of these species subcomplejo were hybridization.
  • ISOLATION OF A RECOMBINANT PROTEIN NATIVE AND GIARDIA LAMBLIA ABLE TO BIND FATTY ACIDS.

    Author: Díaz de la Guardia Quiles Rafael.
    Year: 2006.
    University: GRANADA [More theses of this university] [www.ugr.es].
    Place of defense: Facultad de Ciencias.
    Place of preparation: Facultad de Ciencias.
    URL: http://www.kriptia.com/en/CIENCIAS_DE_LA_VIDA/ZOOLOGIA//PARASITOLOGIA_ANIMAL/1#116396
    Summary: Giardia is a flagellate protozoan that infects the upper small intestine tract of many species of vertebrates, which include men, being the most frequent cause of human gastrointestinal infection caused by parasitic protozoa. In this study, we have characterized some of the properties that a protein Giardia lamblia, isolated in our laboratory, which has similar characteristics to a FABP, such as: * Its molecular weight accurate. * Their ability to bind fatty acids, along with the kinetics of these displacement and constant displacement. * The performance in its purification. * The ability to form aggregates of protein, together with the loss of functionality when this happens and possible biological significance. * The proportion of amino acids that make up a fragment of this protein, possessing the extreme N-terminal locked. We have also known the nucleotide sequence that encodes, by conducting various genetic approaches such as: * The amplification of the gene encoding this protein using genomic DNA, and two primers designed a consensus nucleotide sequence of this same protein sequence but from other agencies. * Conducting a reaction 3 premium RACE from mRNA and one primer specific to the consensus sequence along with two nonspecific created in an artificial way. * Conducting a genoteca expression, which we crawled, obtained a positive clone, whose sequence has been analyzed, in addition to finding inside a intrón, leading to discuss the idea now accepted that the introns entered the genome eukaryotic through mitochondria.
  • CONTROL OF FASCIOLIASIS SHEEP: STUDIES ON RESISTANCE TO FASCIOLICIDAS AND DEVELOPMENT OF TECHNIQUES FOR DETECTION AND CONTROL METHODS

    Author: VARA DEL RIO MARÍA DEL PILAR.
    Year: 2006.
    University: LEÓN [More theses of this university] [www.unileon.es].
    Place of defense: FACULTAD DE VETERINARIA.
    Place of preparation: FACULTAD DE VETERINARIA.
    URL: http://www.kriptia.com/en/CIENCIAS_DE_LA_VIDA/ZOOLOGIA//PARASITOLOGIA_ANIMAL/1#118452
    Summary: The sheep production in Castilla y Leon has been and remains a great significance within livestock production. The fascioliasis is a parasitic disease caused by trematodes gender Fauciola, stressing F. Hepatic as one of the most common species and distribution cumopolita, being the sheep of the most affected species and their presence being linked to environmental conditions that influence both the development of its intermediate stages as the intermediate host. The administration of fasciolicidas and technique more often fight the disease, and we have already described the initial resistance to the most common treatments: albendazole, triclabendazol and clorsulón. The objectives of this work are to determine the prevalence of fascioliasis in the Community as well as the possible presence of resistance and to develop technologies to detect both types of molecular and enzyme or other. There has been monitoring the presence of eggs in feces and evaluation of various parameters enzyme, hemóticos and biochemical. We have designed a model of sampling flocks based inthe initial deworming and sampling that are repeated day +5, +15, +30 Prevalence of Community has been and 26.6% of herds (greater than previous studies) . A 50% holdings of any one drug was not effective in 3 of which 2 were not and 1 last none were elicaz. The infection hosts intemedios and final show less adaptation. We have found differences at the level of gene coding for the subunit 285 ribosumal and the B-tubulina.
14 theses in 1 pages: 1
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