IN VITRO BIOAVAILABILITY OF CALCIUM, IRON AND ZINC IN ENRICHED MILK AND MILK-BASED PRODUCTS.Author:
PERALES AGUSTÍN SARA.
Year: 2005.
University:
VALENCIA.
Place of defense: FACULTAD DE FARMACIA.
Place of preparation: FACULTAD DE FARMACIA (UNIVERSIDAD DE VALENCIA-ESTUDI GENERAL).
Summary: The food is the most important factor extrinsic to the proper nutritional status of children. In the neonatal period breast milk and / or infant formulas are the only food they eat babies and should meet their nutritional needs, with mineral elements essential for development. From a nutritional standpoint interested in knowing the mineral content of foods, and the fraction of people who can absorb and use, namely its bioavailability. Since the determination of the mineral bioavailability in vivo studies presented by various drawbacks, in vitro methods are an alternative. They are based on the simulation of a gastrointestinal digestion and the estimated fraction of soluble element or dialysable. In an attempt to get a better approximation to the situation in vivo, have been introduced cell cultures remain cells Caco-2 model validated intestinal epithelium. In this Doctoral Thesis is estimated and compared in vitro bioavailability of calcium, iron and zinc from milk and baby foods, using methods of solubility, dialysis and a combined method of gastrointestinal simulated digestion / cells Caco-2 that allows the capture and evaluate transport criteria estimators as mineral bioavailability. To date there has been no work together to assess the uptake and transport cells Caco-2 of minerals from milk and baby foods, which highlights the importance of this study. The determination of mineral elements is carried out by atomic absorption spectrophotometry (AAS) flame, the advantages over other techniques. However, the EAA also presents a number of disadvantages that lead to the search for methods more sensitive, specific and less laborious for determining the mineral bioavailability. Thus, the determination of intracellular and ferritin content of zinc free cytosolic fluorescence are postulated as alternative to the determination of iron and zinc. Among the parameters that influence the mineral bioavailability are the dietary factors. In this regard, baby food and milk, receive special mention mineral content of the food, vitamin D and antioxidant compounds.
